Recombinant ISR-alpha anticorps (Extracellular Domain) (CF®594)

N° du produit ABIN7655331

Cet anticorps anti-ISR-alpha est un anticorps Lapin Monoclonal détectant ISR-alpha dans IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour Recombinant ISR-alpha anticorps (Extracellular Domain) (CF®594) (ABIN7655331)

Antigène: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ISR-alpha est conjugé à/à la CF®594

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: INSR-2277R

Détail du produit anti-ISR-alpha anticorps (CF®594)

Épitope: Extracellular Domain

Fonction: Insulin Receptor Alpha (INSR/2277R), CF594 conjugate

Attributs du produit: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular alpha subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of extracellular domain of human Insulin Receptor alpha

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Jurkat cells. Human pancreas.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur ISR-alpha

Antigène: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Autre désignation: Insulin Receptor Alpha

Sujet:

Synonyms: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR

Gene Symbol: INSR

Poids moléculaire: 135 kDa

ID gène: 3643

UniProt: P06213